Downloadable  NOTES
The following TECHNICAL INFORMATION are available for download (PDF format)

Rainbow Calibration Particles
Rainbow Fluorescent Particles
Ultra Rainbow Calibration Particles
Ultra Rainbow Fluorescent Particles
Drop Delay Calibration Particles
Fluorescent Alignment Particles
AccuCount Particles
Flow Cytometry Multiplexing Assay Particles
Flow Cytometry Compensation Particles
Fluorescent Particles Info
Polystyrene Particles Info
Magnetic Particles
Magnetic Separators

Sphero PMT QC Template for RCP-30-5A Lot Z02
RCP-30-5A Lot Z02 PMT QC Template

SpheroTECHNICAL NOTES
The following SpheroTECHNICAL NOTES are available for download (PDF format)

STN-1: Particles Coating Procedures
STN-2: Determination of Antibody binding to particles
STN-3: Binding Capacity of Avidin Magnetic Particles
STN-4: Binding Capacity of Gt-anti-Ms-IgG Magnetic Particles
STN-5: Binding Capacity of Streptavidin Magnetic Particles
STN-6: Binding Capacity of Biotin Magnetic Particles
STN-7: Separation of Mononuclear Cells from Peripheral Blood using SPHERO Gt-anti-Ms-IgG Magnetic Particles
STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles
STN-9: Measuring MEF with Flow Cytometer Using SPHERO Rainbow Calibration Particles.
STN-10: Magnetic Particles Enzyme Immunoassay (MPEIA) using UltraMag Separator System (UMS-4000)
STN-11: Magnetic Particles Coated with Pepsin, Papain and Trypsin
STN-12: Protein A Coated Magnetic Particles
STN-15: Measuring Absolute Cell Count Using SPHERO™ ACCUCOUNT Fluorescent Particles

CHARACTERISTICS OF POLYSTYRENE PARTICLES
Density: 1.05
Refractive Index: 1.59
Composition: Linear polystyrene
Shape: Uniform microspheres
Porosity: Nonporous
Compatibility with organic solvent: Inert to alcohol and DMSO but soluble in DMF, acetone, acetonitrile, xylene,     chloroform and methylene chloride.
Functional groups: Located on the surface with alkyl linker arms.

Functional group contents:
0.8 um Carboxyl particles: ~ 50 ueq/g solid
0.8 um Amino particles: ~ 15-20 ueq/g solid

AVIDIN POLYSTYRENE PARTICLES (0.8 um):
Covalently coated with egg white avidin
Avidin Contents : ~ 14 ug/mg solid; ~ 0.212 nmole/mg solid
Binding capacity to Biotin-Fluorescein: ~ 0.46 nmole/mg solid

ANTIBODY COATED POLYSTYRENE PARTICLES (0.8 um):
Antibody contents: ~ 14 ug/mg solid; ~ 0.2 ug/sq cm; ~1.5x104 IgG/particle
Binding capacity to IgG-FITC: ~ 4 ug/mg

VOLUME, NUMBER AND SURFACE AREA VS. PARTICLE SIZE
Volume of particle: V = (3.14/6) x D^3 x 10e-12 cm3/particle
Number of particles: N = (6W/3.14PD^3) x 10e12 particles
Surface area of particles: A = (6W/PD) x 10e4 cm2

Where
W = Weight of polymer in gram
P = Density of polymer (polystyrene = 1.05)
D = Diameter of particles in micrometer

Total surface area of 1 ml of 5% w/v (50 mg) particle:
A = 2857/D cm2
Total surface area of 20 ul of 0.25% w/v (50 ug) particles:
A = 2.857/D cm2

For 1 ml of 1% w/v (10 mg) particles

DIAMETER(um)               SURFACE AREA(cm2)        #(x10e9)
0.05------------------11428-------------------145513
0.10-------------------5714--------------------18189
0.50-------------------1143----------------------145.51
0.80--------------------714-----------------------35.53
1.0---------------------571-----------------------18.19
1.5---------------------381------------------------5.389
2.0---------------------286------------------------2.274
2.5---------------------229------------------------1.164
3.0---------------------190------------------------0.6737
3.5---------------------163------------------------0.4242
4.0---------------------143------------------------0.2842
4.5---------------------127------------------------0.1996
5.0---------------------114------------------------0.1455
5.5---------------------104------------------------0.1093
6.0----------------------95------------------------0.0842
6.5----------------------88------------------------0.06625
7.0----------------------81------------------------0.0530
8.0------------------------------------------------0.03552
10.0-----------------------------------------------0.01819
12.0-----------------------------------------------0.01052
15.0-----------------------------------------------0.00539