FLOW CYTOMETRY ALIGNMENT PARTICLES

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Flow Cytometry Alignment Particles

Spherotech offers a wide variety of particles for determining if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO™Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.

SPHERO™ Ultra Rainbow Fluorescent Particles - A single population of Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
URFP-02-2	Ultra Rainbow Fluorescent Particles	10¹⁰/mL	0.1-0.3 µm	2 mL	$250.00
URFP-05-2	Ultra Rainbow Fluorescent Particles	10⁷/mL	0.4-0.6 µm	2 mL	$250.00
URFP-10-5	Ultra Rainbow Fluorescent Particles	10⁷/mL	1.0-1.4 µm	5 mL	$250.00
URFP-30-2	Ultra Rainbow Fluorescent Particles	10⁷/mL	3.0-3.4 µm	2 mL	$250.00
URFP-30-20	Ultra Rainbow Fluorescent Particles	10⁷/mL	3.0-3.4 µm	20 mL	$2000.00
URFP01-30-2K	Ultra Rainbow Fluorescent Particles	10⁶/mL	3.0-3.4 µm	2x15 mL	$355.00
URFP01-30-10K	Ultra Rainbow Fluorescent Particles	10⁶/mL	3.0-3.4 µm	10x3 mL	$410.00
URFP-38-2	Ultra Rainbow Fluorescent Particles	10⁷/mL	3.5-3.9 µm	2 mL	$255.00
URFP-38-10	Ultra Rainbow Fluorescent Particles	10⁷/mL	3.5-3.9 µm	10 mL	$1000.00
URFP-38-5A	Ultra Rainbow Fluorescent Particles, Mid-Range	10⁷/mL	3.5-3.9 µm	5 mL	$565.00
URFP-100-2	Ultra Rainbow Fluorescent Particles	10⁷/mL	8.1-12.0 µm	2 mL	$285.00
URFP-150-2	Ultra Rainbow Fluorescent Particles	5x10⁶/mL	13.0-17.9 µm	2 mL	$285.00
URFP-200-5	Ultra Rainbow Fluorescent Particles	1.0% w/v	18.0-24.9 µm	5 mL	$290.00
URFP-300-5	Ultra Rainbow Fluorescent Particles	1.0% w/v	25.0-35.0 µm	5 mL	$290.00

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Ultra Rainbow Fluorescent Particles Product Flyer

Ultra Rainbow Fluorescent Particles

SPHERO™ Rainbow Fluorescent Particles - A single population of Rainbow Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RFP-20-5	Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-20-5)	10⁷/mL	1.8-2.2 µm	5 mL	$245.00
RFP-30-5 (1 peak)	Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5)	10⁷/mL	3.0-3.4 µm	5 mL	$255.00
RFP-30-5A (1 peak)	Rainbow Fluorescent Particles (Intensity similar to mid range FL1 fluorescence in RCP-30-5)	10⁷/mL	3.0-3.4 µm	5 mL	$255.00
RFP-35-5	Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-35-5)	10⁷/mL	3.5-4.0 µm	5 mL	$255.00
RFP-38-5	Rainbow Fluorescent Particles	10⁶/mL	3.8 (+/-0.2)µm	5 mL	$110.00
RFP-50-5	Rainbow Fluorescent Particles	10⁷/mL	5.0-5.9 µm	5 mL	$265.00
RFP-60-5	Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-60-5) (Recommended for FL1, FL2, and FL3 channels only)	10⁷/mL	6.0-6.4 µm	5 mL	$265.00
RFP-70-5	Rainbow Fluorescent Particles	10⁷/mL	6.5-8.0 µm	5 mL	$265.00
RFP-100-2	Rainbow Fluorescent Particles	10⁷/mL	8.1-12.0 µm	2 mL	$210.00

Downloadable NOTE

Rainbow Fluorescent Particles

SPHERO™ Supra Rainbow Midrange Fluorescent Particles - Consists of a single population which has an intensity similar to cellular samples with excellect CVs in all channels - Excellent for instrument set-up at the same fluorescent settings as the analysis of cellular samples
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
SRCP-35-2A	Supra Rainbow Midrange Fluorescent Particles	10⁷/mL	3.0-3.59 µm	2 mL	$240.00
SRCP-35-5A	Supra Rainbow Midrange Fluorescent Particles	10⁷/mL	3.0-3.59 µm	5 mL	$530.00
SRCP01-35-10A	Supra Rainbow Midrange Fluorescent Particles	10⁶/mL	3.0-3.59 µm	10 mL	$115.00

Downloadable NOTE Supra Rainbow Fluorescent Particles

SPHERO™ Fluorescent Alignment Particles - Inexpensive, highly concentrated of Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
FAP-3056-5	Fluorescent Alignment Particles	10⁸/mL	3.0-3.4 µm	5 mL	$635.00

Downloadable NOTE

Fluorescent Alignment Particles

SPHERO™ Ultra Rainbow Fluorescent Particles for Beckman Coulter Cytometers - Consists of a single peak for performance verification of Beckman Coulter flow cytometers
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
B27034	Calibration Beads for MoFlo Astrios	10⁷/mL	3.0-3.4 µm	2 mL	$240.00
B28479	Calibration Beads for MoFlo, MoFlo XDP & Cyan ADP	10⁷/mL	3.0-3.4µm	2 mL	$240.00
ACURFP-38-5	AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL	10⁶/mL	3.8 µm (+/-0.3)	5 mL	$160.00
ACURFP-38-15	AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL	10⁶/mL	3.8 µm (+/-0.3)	15 mL	$395.00

SPHERO™ AccuCount Ultra Rainbow Alignment Particles - Prediluted and enumerated Ultra Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity - Excellent for instrument set-up at the same fluorescent settings as the analysis of cellular samples - Used for instrument set-up and cellular enumeration by Tario J.D., Muirhead K.A., Pan D., Munson M.E., Wallace P.K. (2011) Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry. In: Hawley T., Hawley R. (eds) Flow Cytometry Protocols. Methods in Molecular Biology (Methods and Protocols), vol 699. Humana Press
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
ACURFP-50-10	AccuCount Ultra Rainbow Fluorescent Particles	1.0x10⁶/mL	5.0-5.9 µm	10 mL	$255.00

SPHERO™ Rainbow Alignment Particles - Prediluted Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
RAP-38-5	Rainbow Alignment Particles	1.2x10⁶/mL	3.5-3.9 µm	5 mL	$160.00