Technical Page - Characteristics of Polystyrene Particles

Technical->Characteristics of Polystyrene Particles

Characteristics of Polystyrene Particles

Volume, Number and Surface Area vs. Particle Size

Volume of particle: V = (3.14/6) x D³ x 10^-12 cm³/particle
Number of particles: N = (6W/3.14PD³) x 10¹² particles
Surface area of particles: A = (6W/PD) x 10⁴ cm²

Where
W = Weight of polymer in gram
P = Density of polymer (polystyrene = 1.05)
D = Diameter of particles in micrometer

Total surface area of 1 ml of 5% w/v (50 mg) particle:
A = 2857/D cm²
Total surface area of 20 µl of 0.25% w/v (50 µg) particles:
A = 2.857/D cm²

For 1 ml of 1% w/v (10 mg) particles

DIAMETER(µm)	SURFACE AREA(cm²)			NUMBER (x10⁹)
0.05	------------------	11428	------------------	145513
0.10	------------------	5714	------------------	18189
0.50	------------------	1143	------------------	145.51
0.80	------------------	714	------------------	35.53
1.0	------------------	571	------------------	18.19
1.5	------------------	381	------------------	5.389
2.0	------------------	286	------------------	2.274
2.5	------------------	229	------------------	1.164
3.0	------------------	190	------------------	0.6737
3.5	------------------	163	------------------	0.4242
4.0	------------------	143	------------------	0.2842
4.5	------------------	127	------------------	0.1996
5.0	------------------	114	------------------	0.1455
5.5	------------------	104	------------------	0.1093
6.0	------------------	95	------------------	0.0842
6.5	------------------	88	------------------	0.06625
7.0	------------------	81	------------------	0.0530
8.0	---------------------------------------------			0.03552
10.0	---------------------------------------------			0.01819
12.0	---------------------------------------------			0.01052
15.0	---------------------------------------------			0.00539

Density: 1.05
Refractive Index: 1.59
Composition: Linear polystyrene
Shape: Uniform microspheres
Porosity: Nonporous
Compatibility with organic solvent: Inert to alcohol and DMSO but soluble in DMF, acetone, acetonitrile, xylene, chloroform and methylene chloride.
Functional groups: Located on the surface with alkyl linker arms.

Functional group contents:
0.8 µm Carboxyl particles: ~ 50 µeq/g solid
0.8 µm Amino particles: ~ 15-20 µeq/g solid

Avidin Polystyrene Particle (0.8 µm):

Covalently coated with egg white avidin
Avidin Contents: ~ 14 µg/mg solid; ~ 0.212 nmole/mg solid
Binding capacity to Biotin-Fluorescein: ~ 0.46 nmole/mg solid

Antibody Coated Polystyrene Particles (0.8 µm):

Antibody contents: ~ 14 µg/mg solid; ~ 0.2 µg/cm²; ~1.5x10⁴ IgG/particle
Binding capacity to IgG-FITC: ~ 4 µg/mg