Magnetic Particle Enzyme Immunoassay (MPEIA) is an immunoassay method for the isolation of antibody/antigen complexes on a solid phase surface of magnetic microparticles. MPEIA has been used to automate the measurement of large molecules such as markers associated with cardiac, fertility, cancer, metabolic, hepatitis, and thyroid testing.
1. In the wells of a microEIA plate make serial 2 fold dilutions of rabbit, human or mouse IgG in 1% bovine serum albumin (BSA) adding 100 µL /well. The control wells should receive 100 µL of 1% BSA without IgG.
2. Add 50 µL of a 0.25% (w/v) suspension of Spherotech Cat. No. CM-40-10 magnetic particles coated with antibodies to rabbit, human or mouse IgG. Dilute the particles in buffer consisting of 1% casein hydrolysate and 0.05% Tween 20 in phosphate buffer saline, pH 7.2.