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Flow Cytometry Alignment Particles

Spherotech offers a wide variety of particles for determining if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer. Additiona information can be found at STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles

SPHERO™ Ultra Rainbow Fluorescent Particles - A single population of Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
URFP-02-2
Ultra Rainbow Fluorescent Particles
1010/mL
0.1-0.3 µm
2 mL
$260.00
URFP-05-2
Ultra Rainbow Fluorescent Particles
107/mL
0.4-0.6 µm
2 mL
$260.00
URFP-10-5
Ultra Rainbow Fluorescent Particles
107/mL
1.0-1.4 µm
5 mL
$260.00
URFP-30-2
Ultra Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
2 mL
$260.00
URFP-30-20
Ultra Rainbow Fluorescent Particles
107/mL
3.0-3.4 µm
20 mL
$2045.00
URFP01-30-2K Ultra Rainbow Fluorescent Particles 106/mL 3.0-3.4 µm 2x15 mL $365.00
URFP01-30-10K Ultra Rainbow Fluorescent Particles 106/mL 3.0-3.4 µm 10x3 mL $420.00
URFP-38-2
Ultra Rainbow Fluorescent Particles
107/mL
3.5-3.9 µm
2 mL
$265.00
URFP-38-5A
Ultra Rainbow Fluorescent Particles, Mid-Range
107/mL
3.5-3.9 µm
5 mL
$575.00
URFP-100-2
Ultra Rainbow Fluorescent Particles
107/mL
8.1-12.0 µm
2 mL
$295.00
URFP-150-2
Ultra Rainbow Fluorescent Particles
5x106/mL
13.0-17.9 µm
2 mL
$295.00
URFP-200-5
Ultra Rainbow Fluorescent Particles
1.0% w/v
18.0-24.9 µm
5 mL
$300.00
URFP-300-5
Ultra Rainbow Fluorescent Particles
1.0% w/v
25.0-35.0 µm
5 mL
$300.00
Downloadable NOTES Ultra Rainbow Fluorescent Particles
SDS - Ultra Rainbow Fluorescent Particles
STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles

SPHERO™ Rainbow Fluorescent Particles
- A single population of Rainbow Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RFP-20-5
Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-20-5)
107/mL
1.8-2.2 µm
5 mL
$255.00
RFP-30-5 (1 peak)
Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5)
107/mL
3.0-3.5 µm
5 mL
$265.00
RFP-30-5A (1 peak)
Rainbow Fluorescent Particles (Intensity similar to mid range FL1 fluorescence in RCP-30-5)
107/mL
3.0-3.4 µm
5 mL
$265.00
RFP-35-5
Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-35-5)
107/mL
3.5-4.0 µm
5 mL
$265.00
RFP-50-5
Rainbow Fluorescent Particles
107/mL
5.0-5.9 µm
5 mL
$275.00
RFP-60-5
Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-60-5) (Recommended for FL1, FL2, and FL3 channels only)
107/mL
6.0-6.4 µm
5 mL
$275.00
RFP-70-5
Rainbow Fluorescent Particles
107/mL
6.5-8.0 µm
5 mL
$275.00
RFP-100-2
Rainbow Fluorescent Particles
107/mL
8.1-12.0 µm
2 mL
$220.00
Downloadable NOTE Rainbow Fluorescent Particles
SDS - Rainbow Fluorescent Particles
STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles

SPHERO™ Supra Rainbow Midrange Fluorescent Particles
- Consists of a single population which has an intensity similar to cellular samples with excellect CVs in all channels
- Excellent for instrument set-up at the same fluorescent settings as the analysis of cellular samples
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
SRCP-35-2A
Supra Rainbow Midrange Fluorescent Particles
107/mL
3.0-3.59 µm
2 mL
$250.00
SRCP-35-5A
Supra Rainbow Midrange Fluorescent Particles
107/mL
3.0-3.59 µm
5 mL
$540.00
SRCP01-35-10A
Supra Rainbow Midrange Fluorescent Particles
106/mL
3.0-3.59 µm
10 mL
$125.00
Downloadable NOTE Supra Rainbow Fluorescent Particles
SDS - Supra Rainbow Fluorescent Particles

SPHERO™ Fluorescent Alignment Particles
- Inexpensive, highly concentrated of Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
FAP-3056-5
Fluorescent Alignment Particles
108/mL
3.0-3.4 µm
5 mL
$645.00
Downloadable NOTE Fluorescent Alignment Particles
SDS - Fluorescent Alignment Particles

SPHERO™ Ultra Rainbow Fluorescent Particles for Beckman Coulter Cytometers
- Consists of a single peak for performance verification of Beckman Coulter flow cytometers
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
C62930
Calibration Beads for MoFlo Astrios
107/mL
3.0-3.4 µm
2 mL
$250.00
B28479
Calibration Beads for MoFlo, MoFlo XDP & Cyan ADP
107/mL
3.0-3.4µm
2 mL
$250.00
ACURFP-38-5
AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL
106/mL
3.8 µm (+/-0.3)
5 mL
$170.00
ACURFP-38-15
AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL
106/mL
3.8 µm (+/-0.3)
15 mL
$405.00
Downloadable NOTE Ultra Rainbow Fluorescent Particles for Beckman Coulter Cytometers
SDS - Ultra Rainbow Fluorescent Particles
STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles

SPHERO™ Rainbow Alignment Particles
- Prediluted Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RAP-38-5
Rainbow Alignment Particles
1.2x106/mL
3.5-3.9 µm
5 mL
$170.00
Downloadable NOTE Rainbow Alignment Particles
SDS - Rainbow Alignment Particle