FLOW CYTOMETRY CALIBRATION PARTICLES

Products->FLOW CYTOMETRY QUANTITATIVE PARTICLES

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Ultra Rainbow & Supra Rainbow Quantitative Particle Kits

The SPHERO™ Ultra Rainbow Quantitative Particle Kit (URQP) contains 6 intensities of Ultra Raibow Beads while the Supra Rainbow Quantitative Particle Kit (SRQP) contains 4 intensities. Both products have been ERF (Equivalent Number of Reference Fluorophores) by NIST. These beads are designed to accurately measure comparable data from different types of instruments at different locations. In addition, they can be used for routine calibration of flow cytometers. By using these particles, the sensitivity, linearity, and component performance of the system can be determined. The sensitivity of the instrument will help detect any dye or light contamination. They can be used to check the instrument's ability to distinguish dim peaks from blank beads. In addition,they can aid in the measurement of the linearity of the instrument to determine if the logarithmic amps and detectors are functioning properly. Finally, the URQP & SRQP can be used to detect laser fluctuations, failed electronics, or obstructions in the flow cell.

SPHERO™ Ultra Rainbow Quantitative Particles (URQP) Supra Rainbow Quantitative Particle Kit (SRQP) - URQP contains 6 intensities of Ultra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laser-based CCD fluorimeter - Provides microsphere reference standards for fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC) and Pacific Blue (PB) fluorescent channels using four different NIST reference fluorophores; fluorescein, Nile Red, APC and Coumarin 30 - Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications. SPHERO™ Supra Rainbow Quantitative Particle Kit (SRQP) - SRQP contains 4 intensities of Supra Rainbow Fluorescent Beads with NIST assigned ERF (Equivalent Number of Reference Fluorophores) values based on a published procedure using NIST SRM 1934 and a calibrated laser-based CCD fluorimeter - Provides microsphere reference standards for fluorescent channels with emission ranges from 425 to 810nm using seven different NIST reference fluorophores; fluorescein, Nile Red, APC, Coumarin 30, Pacific Orange, Alexa Fluor 700 & Alexa Fluor 750 - Used during flow cytometry standardardization and calibration measurements for the further advancement of biological and clinical applications .
Catalog No.	Description	Concentration	Nominal Size	Package Size	Price
URQP-38-6K	Ultra Rainbow Quantitative Particle Kit, 6 Intensities, (NIST Traceable ERF Flow Cytometry Standard)	2E6/mL	3.5-3.9 µm	6x2 mL	$255.00
SRQP-38-4K	Supra Rainbow Quantitative Particle Kit, 4 Intensities, (NIST Traceable ERF Flow Cytometry Standard)	2E6/mL	3.5-3.9 µm	4x2 mL	$250.00

Downloadable NOTES:
Product details: Ultra Rainbow Quantitative Particle Kit
URQP NIST: SRM 1934 Certificate
SRQP NIST: SRM 1934 Certificate

References:
- Wang, L. and Gaigalas, A., (2011). “Development of Multicolor Flow Cytometry Calibration Standard: Assignment of Equivalent Reference Fluorophores (ERF) Unit.” J. Res. Natl. Inst. Stand. Technol. 116, 671-683
- Wang, L., Gaigalas, A.,and DeRose, P., (2016). “Assignment of the Number of Equivalent Reference Fluorophores to Dyed Microspheres.” J. Res. Natl. Inst. Stand. Technol., 121, 264-281
- Wang, L., Degheidy, H., Abbasi, F., Mostowski, H., Marti, G., Bauer, S., Hoffman, R.A., and Gaigalas, A.K. 2016. Quantitative flow cytometry measurements in antibodies bound per cell based on a CD4 reference. Curr. Protoc. Cytom. 75:1.29.1-1.29.14. doi: 10.1002/0471142956.cy0129s75
- Wang, L., Stebblings, R., Gaigalas, A., et al, (2015). "Quantification of Cells with Specific Phenotypes II: Determination of CD4 Expression Level on Reconstituted Lyophilized Human PBMC Labelled with Anti-CD4 FITC Antibody." Cytometry Part A 87A, 254-261
- Wang, L., Gaigalas, A.K., Marti, G.E., Abbasi, F. and Hoffman, R.A. (2008) Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry. Cytometry Part A, 73A, 279-288. http://dx.doi.org/10.1002/cyto.a.20507