SpheroTechnical Note #2 - Determination of Antibody Binding to Particles

The amount of the antibody binding to the particles after coating can be determined by using the
subtraction method as follows:
1. Measure the absorbance of antibody solution to be used for
coating at 280 nm (OD280). The IgG has OD280 of ~1.4/mg.
2. Pellet the particles to be coated by centrifugation or magnetic

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