SpheroTechnical Notes #21 - SPHERO Streptavidin Particle Uses and Protocols

Streptavidin is a protein (MW of approx. 66,000) made up of four identical subunits, each containing a high affinity binding site for biotin (KD = 10-15 M). It has the same biotin binding properties as avidin, but less non-specific binding is observed. It has been used both in immune assays and genomic assays for target detection.

Spherotech Streptavidin bead surfaces are designed as a matrix for simple and efficient methods such as:
- Protein coated beads for the isolation of biotinylated compounds such as proteins, immunoglobulins, sugars, lectins or DNA/RNA and microRNA
- Magnetic streptavidin coated beads can be used as substrates for both immune assays and genomic assays
- Small fluorescently labeled streptavidin particles can be used as probes for detection.
Select the link below to download the complete SpheroTECHNICAL NOTE. (PDF format)

SpheroTechnical Note #21 will provide the following information:
  • Instructions for the preparations of SPHERO Streptavidin Particles before use
  • Optimized biotinylation protocols for various ligands in order to facilitate optimal binding to An introduction to SPHERO Streptavidin Coated Beads capture antibodies with conjugated fluorophores to provide detectable signals.
  • Protocols for the immobilization of biotinylated nucleic acids, antibodies, and PEG-biotins
  • Methods to quantitative the amount of biotinylated DNA after binding to SPHERO Streptavidin Coated Beads