SpheroTechnical Notes #22 - SPHERO Goat anti-Mouse Particle Uses and Protocols

Since the 1950’s, latex based immunoassays have been used in clinical laboratories. The first described latex agglutination assay was developed by Plotz and Singer as a Rheumatoid Factor test in 1956. Currently, in the life sciences immunoassays are used for detecting different proteins, hormones, and antibodies. .

Spherotech Goat anti-Mouse bead particles are designed for:
- The improvement of Sandwich Assay development by optimizing the orienatation of monoclonal antibodies for site-specific immobilization of antigens
- Reducing the complexity of Magnetic Immunoprecipitation using Goat anti-Mouse IgG (Fc) Magnetic Beads
Select the link below to download the complete SpheroTECHNICAL NOTE. (PDF format)

SpheroTechnical Note #22 will provide the following information:
  • Information on how to use Spherotech antibody coated beads in sandwich immunoassays
  • Protocols for developing a bead based assay
  • Protocols for washing Spherotech antibody coated beads and coating with monoclonal antibodies
  • Protocols for the Magnetic Immunoprecipitation using Goat anti-Mouse IgG (Fc) Magnetic Bead
  • Protocols for regeneration and reuse of Spherotech antibody coated beads