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SpheroTechnical Notes #22 - SPHERO Goat anti-Mouse Particle Uses and Protocols
 

Introduction
Since the 1950’s, latex based immunoassays have been used in clinical laboratories. The first described latex agglutination assay was developed by Plotz and Singer as a Rheumatoid Factor test in 1956. Currently, in the life sciences immunoassays are used for detecting different proteins, hormones, and antibodies. .

Spherotech Goat anti-Mouse bead particles are designed for:
- The improvement of Sandwich Assay development by optimizing the orienatation of monoclonal antibodies for site-specific immobilization of antigens
- Reducing the complexity of Magnetic Immunoprecipitation using Goat anti-Mouse IgG (Fc) Magnetic Beads
Select the link below to download the complete SpheroTECHNICAL NOTE. (PDF format)


SpheroTechnical Note #22 will provide the following information:
  • Information on how to use Spherotech antibody coated beads in sandwich immunoassays
  • Protocols for developing a bead based assay
  • Protocols for washing Spherotech antibody coated beads and coating with monoclonal antibodies
  • Protocols for the Magnetic Immunoprecipitation using Goat anti-Mouse IgG (Fc) Magnetic Bead
  • Protocols for regeneration and reuse of Spherotech antibody coated beads
  •