MATERIALS:
1. Streptavidin magnetic particles, 1% w/v, Cat. # SVM-40-5 Lot No. Y01, 4.35 µm
2. Biotin-FA solution, Spherotech, Lot No. 021688, 533 nM in 1% diluent (IBS containing 1% normal goat serum and 1% fetal bovine serum )

PROCEDURES:
1. Adjust the fluorimeter for excitation and emission at 490 and 520 nm respectively.
2. Set 100% emission with the Biotin-FA solution.
3. Add 50, 100, 150, 200, 300, and 400 µL of Streptavidin magnetic particles to six 1.5 ml microfuge tubes.

Select the link below to download the complete SpheroTECHNICAL NOTE. (PDF format)