MATERIALS:
1. Biotin magnetic particles, 1% w/v, Cat. # TM-40-10 Lot No. J01, 4.32 µm
2. Avidin-FITC solution, Jackson ImmunoResearch, #003-090-083, Lot No. 21452, 5 µg/mL in 1% diluent.(IBS containing 1% normal goat serum and 1% fetal bovine serum )

PROCEDURES:
1. Adjust the fluorimeter for excitation and emission at 490 and 520 nm respectively.
2. Set 100% emission with the Avidin-FITC solution.
3. Add 25, 50, 75, 100 and 200 µL of Biotin magnetic particles to five 1.5 mL microfuge tubes.
4. Separate the particles magnetically and remove the supernatant.
5. Add 1 mL of Avidin-FITC solution to each tube, vortex and rotate at room temperature for one hour.

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